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Registros recuperados: 59
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Aplicación de plantas medicinales para la antisepsia de pezones de vacas posordeño Plantas Medicinales
Almeida Schiavon,Diane Bender; Damé Schuch,Luiz Filipe; Bastos Oyarzabal,Marta Elaine; de Souza Prestes,Luciana; Zani,João Luiz; de Andrade Hartwig,Carla.
Introducción: el sistema de producción de leche agroecológico está limitado por alternativas sostenibles de promoción a la salud animal. El control de la mastitis con plantas medicinales puede ser viable de modo económico y ecológicamente. Objetivos: evaluar la aplicación de antisépticos en la desinfección de pezones posordeño. Métodos: se comparó un convencional y extracto de plantas medicinales en un rebaño comercial y tuvo como principal encierre las nuevas infecciones intramamarias. Resultados: la prevalencia semanal del California Mastitis Test varió en el grupo 1 entre 29,5 y 17,1 %, y en el grupo 2 de 29,7 a 19,6 %, no diferenciándose de modo significativo, en ninguna de las semanas. La incidencia de cultura positiva para...
Tipo: Journal article Palavras-chave: Mastitis; Plantas medicinales; Tagetes minuta.
Ano: 2011 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962011000300005
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Comparative analysis of agr groups and virulence genes among subclinical and clinical mastitis Staphylococcus aureus isolates from sheep flocks of the Northeast of Brazil BJM
Almeida,Lara M. de; Almeida,Mayra Zilta P.R.B. de; Mendonça,Carla L. de; Mamizuka,Elsa M..
Staphylococcus aureus is one of the most frequent mastitis causative agents in small ruminants. The expression of most virulence genes of S. aureus is controlled by an accessory gene regulator (agr)locus. This study aimed to ascertain the prevalence of the different agr groups and to evaluate the occurrence of encoding genes for cytotoxin, adhesins and toxins with superantigen activity in S. aureus isolates from milk of ewes with clinical and subclinical mastitis in sheep flocks raised for meat production The agr groups I and II were identified in both cases of clinical and subclinical mastitis. Neither the arg groups III and IV nor negative agr were found. The presence of cflA gene was identified in 100% of the isolates. The frequency of hla and lukE-D...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Agr groups; Virulence genes; Mastitis; Sheep flock.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200026
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Accurate identification of atypical Staphylococcus chromogenes plasma-clotting strains causing bovine mastitis Ciência Rural
Avellar-Costa,Pedro; Santos,Danielle Cabral dos; Lange,Carla Christine; Laport,Marinella Silva; Brito,Maria Aparecida Vasconcelos Paiva; Rossi,Ciro César; Giambiagi-deMarval,Marcia.
ABSTRACT: We compared the potential of routine techniques used for the identification of Staphylococcus species, aiming to evaluate their accuracy in the detection of 43 Staphylococcus chromogenes strains isolated from bovine mastitis that, despite being a coagulase-negative species, are able to clot plasma. These strains could be mistakenly suspected to be S. aureus and lead to an unappropriated treatment of the disease. MALDI-TOF, PCR-RFLP of the chaperonine gene groEL, and sequencing of the 16S rRNA and elongation factor Tu gene tuf were employed. Results from the four methods were coincident for only half of the strains because of the low accuracy of the groEL PCR-RFLP (51.2% accuracy). Even though all the sequencing results were identical, the high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus chromogenes; Mastitis; MALDI-TOF; Tuf; GroEL.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600452
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Relationship between milk production system and milk traits and somatic cell counts in Brazilian Murrah buffaloes: a multivariate analysis Animal Sciences
Barbosa, Severino Benone Paes; Modesto, Elisa Cristina; Lopes, Fabiana de Araújo; Silva, Elizabete Cristina da; Abad, Atzel Cândido Acosta.
The purpose of this study was to evaluate the monthly milk production and quality of buffaloes from two milk production systems in the Brazilian northeast using the multivariate analysis: principal component analysis (PCA). A total of 2,506 individual milk recordings were performed in two production systems, containing information on milk production (kg day-1), fat, protein, lactose and total solids counts and somatic cell count (SCC). There were positive correlations between the fat content and the contents of total solids (TS) and protein, and of TS and protein. From the PCA, two main components (PC1 and PC2) were identified, explaining 67.71% of the total variation. The fat, protein, lactose and ST level, represented by PC1, explain 46.18% of the total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bubalus bubalis; Mastitis; Milk recording; Score of somatic cells..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/46522
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Direct identification of bovine mastitis pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in pre-incubated milk BJM
Barreiro,Juliana R.; Gonçalves,Juliano L.; Grenfell,Rafaella; Leite,Renata F.; Juliano,Luiz; Santos,Marcos V..
ABSTRACT The present study aimed to compare two MALDI-TOF identification methods [(a) direct sample identification after pre-incubation; or (b) use of bacteria isolated on pre-culture)] to standard, traditional bench microbiology. A total of 120 quarter milk samples from 40 Holstein lactating cows were screened based on culture-positive results obtained by microbiological culture (reference method) with the following numbers of quarters positive per cow: 4 cows with 1, 8 cows with 2, 12 cows with 3 and 16 cows with 4 infected quarters per cow. For direct identification method, quarter milk samples (n = 120) were skimmed by centrifugation (10,000 × g/10 min) and pre-incubated at 37 ºC for 12 h. After pre-incubation, quarter milk samples were submitted to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Milk; Bacteria; Mastitis; Mass spectrometry.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400801
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Actividad antimicrobiana de aceite esencial de Origanum vulgare L. ante bacterias aisladas en leche de bovino Plantas Medicinales
Bastos Oyarzabal,Marta Elaine; Damé Schuch,Luiz Filipe; de Souza Prestes,Luciana; Bender Almeida Schiavon,Diane; Alves Rodrigues,Maria Regina; Braga de Mello,João Roberto.
Introducción: la mastitis bovina es el mayor problema en la producción lechera, causada principalmente por bacterias grampositivas. La búsqueda de principios activos que actúen en esos microrganismos es creciente, sobre todo por la ocurrencia de multirresistencia bacteriana. Objetivos: evaluar la concentración bactericida mínima del aceite esencial de Origanum vulgare L. (orégano) frente a bacterias aisladas de leche mastítica. Métodos: se evaluó la actividad antimicrobiana del aceite esencial de O. vulgare frente a 71 bacterias aisladas de leche bovina, de los géneros Streptococcus, Staphylococcus y Corynebacterium; y 3 cepas patrón de Pseudomonas aeruginosa, Staphylococcus aureus y Escherichia coli. La técnica utilizada fue de dilución en microplaca....
Tipo: Journal article Palavras-chave: Origanum vulgare; Mastitis; Antimicrobiano.
Ano: 2011 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962011000300006
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Mammary botryomycosis: two concomitant cases in mares: case report Arq. Bras. Med. Vet. Zootec.
Belli,C.B.; Fonte,J.S.; Torres,L.N.; Gondin,M.R.; Maiorka,P.C.; Fernandes,C.B..
ABSTRACT Mammary disorders in mares are rare and usually only one animal per paddock is affected. In this report, two mares with 7 and 9 years old, were concomitantly diagnosis of chronic pyogranulomatous mastitis, associated with the Splendore-Hoeppli reaction, indicative of botryomycosis a rare, chronic suppurative disease with microabscess formation, characterized by in vivo formation of eosinophilic materials around microorganisms or biologically inert material. Various bacteria can cause botryomycosis in horses, and the most frequently isolated one is Staphylococcus sp., particularly S. aureus. This report confirms the role of Staphylococcus sp.; however, specifically S. hyicus and S. cohnii that prior to the current report, had not been associated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Equine; Mastitis; Mammary gland; Botryomycosis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000200342
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CONTROL INTERNO DE LA CALIDAD APLICADO AL CENMAST, PRODUCTO PARA DETECTAR MASTITIS Rev Salud Anim.
Betancourt,A; Ramírez,Anledys; Navarro,Verónica; González,Daisy; López,Yanet; Linares,Anel.
El CENMAST es un diagnosticador con un principio semejante a la prueba de California, durante su producción por el Centro Nacional de Sanidad Agropecuaria, el laboratorio de control de la calidad comprueba las especificaciones del producto, con las determinaciones del principio activo, pH y densidad, cuyos resultados están bajo un programa de control interno de la calidad. Se evaluó un lote del diagnosticador como material de referencia de trabajo, para el uso como muestra de control de la calidad, también se empleó el mismo en la validación de la precisión de los ensayos en condiciones de repetibilidad y condiciones intermedias variando los analistas, día de trabajo, equipos y la calibración. Se halló una adecuada precisión entre las réplicas, se...
Tipo: Journal article Palavras-chave: Control interno de la calidad; Diagnosticador; Mastitis.
Ano: 2010 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2010000300004
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Lactation stage and udder health status of Santa Ines ewes Arq. Bras. Med. Vet. Zootec.
Blagitz,M.G.; Benites,N.R.; Melville,P.A.; Batista,C.F.; Betiol,P.S.; Azedo,M.R.; Gomes,V.; Souza,F.N.; Della Libera,A.M.M.P..
Avaliou-se o risco de infecção em diferentes fases da lactação em 33 ovelhas da raça Santa Inês. Inicialmente a glândula foi submetida ao exame físico e à prova de fundo escuro. Posteriormente, amostras de leite foram coletadas assepticamente para a realização de exame bacteriológico - California Mastitis teste -, e contagens microscópica e automática de células somáticas. Nenhuma diferença foi observada entre as distintas fases de lactação. Observou-se alta persistência de infecções intramamárias, e tendência a maior contagem de células somáticas no último período de lactação, que pode ser oriunda da maior resistência a infecções neste período.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ewe; Somatic cell count; Lactation stage; Mastitis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200034
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Controle integrado de mastite bovina. Infoteca-e
BRITO, J. R. F.; BRESSAN, M..
bitstream/item/132469/1/Livro-Controle-integrado-da-mastite-bovina.pdf
Tipo: Livro técnico (INFOTECA-E) Palavras-chave: Mastite; Integrated control.; Controle Integrado; Leite.; Mastitis; Milk..
Ano: 1996 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/592224
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Mammary adenocarcinoma in a mare Ciência Rural
Brito,Marilene de Farias; Seppa,Gilberto Santos; Teixeira,Luisa Gouvêa; Rocha,Thais Gomes; França,Ticiana do Nascimento; Hess,Tanja Maria; Peixoto,Paulo Vargas.
A non-lactating 17-year-old grey barren Mangalarga Marchador mare was referred to the Large Animal Veterinary Hospital of the Federal Rural University of Rio de Janeiro (UFRRJ), presenting enlargement of the mammary gland. The owner reported that the animal had a lesion in the mammary gland for at least two years, diagnosed and treated as chronic mastitis. Initially only the right gland was involved, presenting ulceration and exudation on the cutaneous surface. After 20 months, the left gland also became affected. The diagnosis of tubulo-papillary adenocarcinoma was based on the typical microscopic lesions. This work shows the importance of the histopathological examination in the differential diagnosis between the neoplasms and the chronic inflammation in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Equine diseases; Mammary neoplasms; Mastitis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000200045
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Determinación de biomarcadores moleculares en leche y su posible aplicación en el diagnóstico y tratamiento de la mastitis bovina InVet
Caggiano,N; Bottini,J.M; Lorenzo Smirnoff,A; De Simone,E.A; Chiappe Barbará,M.A.
La mastitis es la patología con mayor morbilidad y mortalidad dentro de los rodeos lecheros, afectando seriamente a la producción láctea y la calidad de la leche. Es por eso que en este trabajo se evaluó la actividad de las Metaloproteasas 2 y 9, involucradas en la degradación de la matriz extracelular, en leche de vacas y vaquillonas con mastitis clínica y subclínica y en animales sanos. Se evaluaron también distintas fracciones proteicas y la actividad caseinolítica total. Las fracciones proteicas se evaluaron mediante SDS-PAGE y a actividad de las Metaloproteasas 2 y 9 fue evaluada mediante zimografía al igual que la actividad caseinolítica total. Los resultados arrojaron en la actividad caseinolítica total diferencias significativas entre el grupo...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mastitis; Metaloproteasas; Caseína.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982016000200004
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TSST-1, enterotoxin and bacteriocin-like substance production by Staphylococcus aureus isolated from foods Arq. Bras. Med. Vet. Zootec.
Carvalho,S.A.; Carmo,L.S.; Abreu,E.F.; Dias,R.S.; Apolônio,A.C.M.; Santos,S.G.; Farias,L.M.; Carvalho,M.A.R..
The production of Toxic Shock Syndrome Toxin-1 (TSST-1), enterotoxins and bacteriocin-like substances was evaluated in 95 strains of Staphylococcus aureus recovered from raw bovine milk (n=31) and from food samples involved in staphylococcal food poisoning (n=64). Enterotoxigenicity tests with the membrane over agar associated to optimal sensibility plate assays were performed and showed that 96.77% of strains recovered from milk and 95.31% from food samples produced enterotoxins A, B, C, D or TSST-1. Reference strains S. epidermidis, Bacillus cereus, Listeria monocytogenes, Lactobacillus casei, Pseudomonas aeruginosa, S. aureus, Salmonella Typhimurium, Escherichia coli, Enterococcus faecalis and Bacteroides fragilis were used as indicator bacteria in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Mastitis; Toxigenicity; Food poisoning; Bacteriocin.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500035
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Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis BJM
Ciftci,Alper; Findik,Arzu; Onuk,Ertan Emek; Savasan,Serap.
This study aimed to detect methicillin resistant and slime producing Staphylococcus aureus in cases of bovine mastitis. A triplex PCR was optimized targetting 16S rRNA, nuc and mecA genes for detection of Staphylococcus species, S. aureus and methicillin resistance, respectively. Furthermore, for detection of slime producing strains, a PCR assay targetting icaA and icaD genes was performed. In this study, 59 strains were detected as S. aureus by both conventional tests and PCR, and 13 of them were found to be methicillin resistant and 4 (30.7%) were positive for mecA gene. Although 22 of 59 (37.2%) S. aureus isolates were slime-producing in Congo Red Agar, in PCR analysis only 15 were positive for both icaA and icaD genes. Sixteen and 38 out of 59 strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; IcaA; IcaD; Slime; Mastitis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200009
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Characterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazil: the need to identify to species level to avoid misidentification Arq. Bras. Med. Vet. Zootec.
Coimbra-e-Souza,V.; Brito,M.A.V.P.; Chamon,R.C.; Laport,M.S.; Giambiagi-deMarval,M..
ABSTRACT Mastitis is an inflammation of the mammary gland that affects dairy cattle worldwide causing economic losses. Coagulase-negative staphylococci (CNS) are the predominant cause of this type of infection. We have recently showed that coagulase-positive staphylococci could be misidentified. So, the aim of this study was to characterize the Staphylococcus spp. strains initially classified as coagulase-negative Staphylococci, isolated from buffalo with subclinical mastitis. Milk of buffaloes with mastitis in herds was collected and 9 strains were identified as CNS by phenotypic tests. Molecular methodologies latter identified the strains as coagulase-negative Staphylococcus chromogenes (5), coagulase-positive Staphylococcus hyicus (2) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Buffalo; Mastitis; Staphylococcus; Milk; Coagulase test.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601669
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The importance of mastitis in dairy production in Brazil MV&Z
Costa, Elizabeth Oliveira da.
Mastitis is an inflammation of the mammary gland that causes severe economic losses due to reduced milk production, expence on pharmaceuticals, veterinary care, discarding of milk after treatment and discarding of affected animals. This inflammatory process may have distinct origins, but all cases of economic importance are caused by microorganisms. According to their origin and means of transmission, the main etiological agents were put together in two groups, contagious microorganisms (Streptococcus agalactiae, Staphylococcus sp, e Corynebacterium bovis) transmitted mainly during rnilking and environmental microorganisms (Streptococcus uberis, Enterobacteriaceae, fungos, algae of the genus Prototheca, etc.).Clinical mastitis is characterized by visible...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mastitis; Bovine; Mammary gland; Microorganisms; Control mastite; Bovinos; Glândula mamária; Microrganismos; Controle.
Ano: 1998 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3381
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Detección de diferentes subtipos genéticos de Mycoplasma bovis en Argentina InVet
Estanguet,A.A.; Tamiozzo,P.J.; Tirante,L.; Chaves,J.; Raviolo,J.; Giraudo,J.; Ambrogi,A..
Mycoplasma bovis es el mycoplasma causante de mastitis más importante en vacas lecheras y ha sido diagnosticado en nuestro país así como a nivel mundial. Para realizar estudios epidemiológicos de brotes, el análisis por MLVA parece ser una herramienta adecuada para la tipificación genética del patógeno. En el presente trabajo se analizaron 7 cepas de M. bovis provenientes de 2 tambos distintos (muestras de leche de tanque de enfriado, y cuartos mamarios de vacas con y sin signos de mastitis) que habían sido previamente identificadas. Se estudiaron por PCR 4 regiones que comprenden secuencias repetidas en tándem (TR). Dentro de los loci TR analizados, sólo se encontró variabilidad en TR 52, en el cual se observaron 2 posibles alelos en muestras que...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ganado lechero; Mastitis; Mycoplasma bovis; Subtipos genéticos; MLVA..
Ano: 2015 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982015000200003
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Occurrence of Staphylococcus aureus in raw milk produced in dairy farms in São Paulo state, Brazil BJM
Fagundes,Helena; Barchesi,Luciana; Nader Filho,Antonio; Ferreira,Luciano Menezes; Oliveira,Carlos Augusto Fernandes.
The objective of the present study was to evaluate the occurrence of Staphylococcus aureus in milk produced in 37 farms located in the regions of Ribeirão Preto and São Carlos, state of São Paulo, Brazil. Two-hundred and eight samples of milk from individual cows showing subclinical mastitis, and 37 samples of bulk tank milk were analyzed. S. aureus strains were detected in 18 (7.3%) milk samples: 14 (6.7%) from samples of individual cows, and 4 (10.8%) from bulk tank milk. Two individual milk samples (14.3%) and two bulk milk samples contained enterotoxigenic S. aureus. PFGE analysis revealed the genetic heterogeneity of the strains isolated from raw milk, which presented to 13 S. aureus patterns. Results confirmed the potential transmission of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Raw milk; Bovine; Mastitis; Enterotoxin.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200018
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Reducción de la mastitis subclínica con el empleo de implante de catgut en el punto de acupuntura Estómago 29 Rev Salud Anim.
Farray González,Jesús Francisco; Rubio García,Juan Luís; Fabré Rodríguez,Yolexis; Alonso Rodríguez,Julio César; Suárez Fernández,Yolanda E..
Se realizó un estudio observacional de diseño transversal en la población bovina del Rancho Lupita, Aguascalientes, México, en el periodo junio-julio de 2013, con el propósito de reducir la prevalencia de mastitis e incrementar la recuperación de cuartos y animales, a través del empleo de acciones y procedimientos de prevención y control. Para determinar la prevalencia, se diagnosticó la mastitis por examen físico individual, mediante la prueba de California, por el conteo de células somáticas y por aislamiento bacteriológico. Para la reducción de la mastitis se instauraron medidas preventivas y de control de la enfermedad y se evaluó la recuperación de los cuartos y animales afectados por medio del implante de catgut en el punto acupuntural E-29. Se logró...
Tipo: Journal article Palavras-chave: Mastitis; Implante; Vacas; Estómago 29; Mastitis; Implant; Cows; Stomach 29.
Ano: 2015 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000200003
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Incidencia y costo de la Mastitis en un establo del Municipio de Santa Ana, Sonora AgEcon
Gerlach B., Francisco Arturo; Ayala Alvarez, Felix; Denogean Ballesteros, Francisco G.; Moreno Medina, Salomon; Gerlach B., Luis Ernesto.
With the objective to know the mastitis cost for milk producer, was developed this study, with duration of a year in a milk farm of the North region of Sonora. The cost of the clinical and subclinical mastitis was estimated, taking into account the milk production, treatment costs and commercial milk value. The information was recorded monthly, the incidence of subclinical mastitis was obtained using California MastitisTest (CMT) and clinical mastitis incidence was obtained from farm health records. Was an incidence average of 18,3% of subclinical mastitis, whereas the incidence of the clinical form of mastitis was of 5,35%. The average monthly cost of each animal with mastitis was $185.40; altogether the mastitis had a cost of $30.966,34 annually,...
Tipo: Journal Article Palavras-chave: Milk; Mastitis; Diseases costs; Dairy cattle; Agribusiness.
Ano: 2009 URL: http://purl.umn.edu/48607
Registros recuperados: 59
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